quantitative methylation massarray epityper  (Sequenom)

Journal: The Indian Journal of Medical Research

Article Title: Promoter-associated DNA methylation & expression profiling of genes ( FLT 3, EPB41L3 & SFN ) in patients with oral squamous cell carcinoma in the Khasi & Jaintia population of Meghalaya, India

doi: 10.4103/ijmr.IJMR_620_18

Figure Lengend Snippet: List of primers for the selected sets of genes used in the MassARRAY ® EpiTYPER

Article Snippet: Quantitative methylation MassArray EpiTyper (EpiTYPER® Agena Bioscience, CA, USA) was carried out for the selected genes, viz., FLT3, EPB41L3 and SFN for methylation analysis of its promoter region-associated CpG island.

Techniques: Sequencing, Amplification

Journal: Oncotarget

Article Title: DNA methylation promotes paired box 2 expression via myeloid zinc finger 1 in endometrial cancer

doi: 10.18632/oncotarget.12626

Figure Lengend Snippet: A. Bisulfite sequencing results showed fragment B1 (-764 to -447 bp) of the PAX2 promoter was hypermethylated in endometrial cancer cell lines and tissues: rows represent clones (10 for each sample), columns represent CpG sites. Black squares represent methylated CpGs, and white squares represent unmethylated CpGs. EEC: endometrial epithelial cell. EnCa: endometrial cancer. N: normal. B. MassARRAY results indicate that PAX2 was hypermethylated in endometrial cancer tissues compared with normal endometrial tissues. M1 is an amplicon of a 280-bp fragment from -723 bp to -443 bp; M2 is an amplicon of a 379-bp fragment from -468 bp to -89 bp. Hypermethylated CpG sites were centralized in the 5’ of M1 (CpG 1 to 7).

Article Snippet: The PCR annealing Tm was 56°C, and sample preparation was performed according to “Training Instructions for EpiTYPER Quantitative Methylation Analysis Using MassCLEAVE for MassARRAY” (Sequenom).

Techniques: Methylation Sequencing, Clone Assay, Methylation, Amplification